Introduction Mycobacterium tuberculosis 1 2,3 M. tuberculosis 4,5 l d d 5 6 Escherichia coli Fig. 1 M. tuberculosis 7 Streptomyces coelicolor M. tuberculosis 8 glnD Materials and methods Culture M. tuberculosis 2 4 2 4 4 2 4 4 9 4 2 4 4 2 4 l Quantitative RT-PCR sigA amt glnB glnD Table 1 sigA amt, glnB glnD sigA glnB glnD amt sigA glnD 10 glnD Kpn Eco Eco Hin Kpn Hin glnD hyg lacZ sacB PacI 10 M. tuberculosis 11 Xho GS assays 12 13 Virulence assays 14 5 6 Results glnD M. tuberculosis 8,15 Fig. 1 glnD amt glnB Fig. 3A amt glnB glnB glnD sigA Fig. 2 Amt glnB glnD glnD glnD glnD hyg lacZ sacB 10 Fig. 3 9 Control of total GS activity 16 Fig. 4 glnD M. tuberculosis 6 glnA1 glnA2 9 Virulence in macrophages and SCID mice M. tuberculosis glnA1 7 glnD Fig. 5 Fig. 5 Discussion glnB amt Corynebacterium glutamicum 17,18 M. tuberculosis 8,15 glnB glnD glnD 6 19 glnD glnA1 glnD M. tuberculosis glnA1 7 M. tuberculosis glnD 7 glnD glnD in vivo glnD M. tuberculosis. glnD