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\begin{document}$$ {\text{NH}}^{{\text{ + }}}_{{\text{4}}}  $$\end{document} 1 3 3 − 4 5 6 10 − 11 12 te i + te i − + 12 2+ i 13 − 14 15 16 First 1 13 17 Second 1 86 18 Third 18 Fourth 1 2+ i 2 2 19 20 2+ 2+ 15 21 2 15 21 15 15 21 15 21 15 21 Methods Cell culture 2+ 2+ 3 2 NKCC measurement − 4 2 D − − 2 2 D 86 86 2 86 + V = A/amt A a 86 + m t 86 Western blotting − Chemicals 1 86 D Statistics t t p Results − 13 − 1 − Fig. 1 Time dependence of NKCC inhibition in C11 cells triggered by the addition of 100 μM ATP  2 15 16 21 Fig. 2 Representative blots showing the time-dependent action of 100 μM ATP, 10 μM forskolin, and 0.1 μM PMA on SAPK phosphorylation in C11 cells  3 4 4 Fig. 3 a b c d −  Fig. 4 a b − a b  50 22 23 23 22 3 5 3 Fig. 5 −  22 23 50 24 Discussion 25 27 First Second 28 50(ATP) 29 30 31 32 33 34 36 33 37 39 38 20 40 Staphylococcus aureus 41 33 42 43 13 17 1 2 44 1 − 2 − 17 2 − 2+ 14 6 + \documentclass[12pt]{minimal}
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\begin{document}$$ {\text{NH}}^{{\text{ + }}}_{{\text{4}}}  $$\end{document} 0.5 45 4 + \documentclass[12pt]{minimal}
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\begin{document}$$ {\text{NH}}^{{\text{ + }}}_{{\text{4}}}  $$\end{document} 2 2+ 1 13 1 6 Fig. 6 − a b 1 − 2 + 3 PLC IP 3 COX PGE 1 1 CAMK 2+ CA a.m. b.m.