Introduction 1 2 Escherichia coli 3 4 6 3 Aeromonas hydrophila 7 Materials and methods Bacterial strains used E. coli E. coli 8 12 13 espF 3 14 sepZ espZ 15 Materials Bacillus cereus N m Bacterial culture E. coli 3 D 3 Cell culture 3 16 6 2 Assay for ecto-5′-nucleotidase by phosphate release i 17 18 i 2 3 2 Cell-bound or monolayer activity E. coli E. coli 19 BIOMOL GREEN detection of phosphate released from AMP i 6 i 1 Detection of CD73 by Western immunoblot 2b 2b 20 Ussing chamber studies sc + − 3 + \documentclass[12pt]{minimal}
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\begin{document}$$ 2.4\;{\text{HPO}}^{2}_{4}  - ,\;0.6\;{\text{H}}_{2} {\text{PO}}^{ - }_{4}  $$\end{document} 2+ 2+ sc 2 2 16 Protein assay 21 Expression of ecto-5′-nucleotidase RNA by reverse transcription and real-time polymerase chain reaction (PCR) 22 t 23 t Data analysis and presentation p Results 1 1 1 M max 6 M max M 24 1 i Fig. 1 i Materials and methods a c d b b a b i c d e f e Ado f g  25 1 1 1 1 4 1 2 E. coli 2 E. coli E. coli E. coli 2 2 2 Fig. 2 E. coli a E. coli b E. coli light gray bars dark gray bars  espF 3 espF 14 26 espF espF p t Fig. 3 a Materials and methods 2 6 b p c m d 6 e f Materials and methods f g sepZ/espZ Lane 1 Lanes 2 and 3 Lanes 4 and 5 sepZ lanes 2 and 3  24 27 28 3 m 29 3 m 3 3 3 3 3 3 1 espF 3 Table 1 Effect of EPEC infection on expression of RNA encoding ecto-5′-nucleotidase in T84 cells, by reverse transcription and real-time PCR Condition a Uninfected control 1.0, by definition +1 U/ml PI-PLC 1.23 ± 0.26 b 0.66 ± 0.22 b c espF b c a b c t p 3 3 sepZ/espZ 4 4 M max 4 max M M 4 50 4 Fig. 4 a b open symbols closed symbols max M c d c d c d 50  4 50 50 5 5 2b 5 sc sc 5 sc 5 5 sc 5 5 5 4 Fig. 5 sc Materials and methods sc a b c sc d sc e e p  3 30 31 6 6 6 6 6 E. coli E. coli 32 33 E. coli 6 Fig. 6 a c b a b c p  Discussion 4 34 2 3 max 27 6 3 1 35 36 E. coli 37 38 Salmonella Entamoeba histolytica 39 Trichinella spiralis 40 Vibrio cholerae 41 42 43 espF 3 espF 19 espF Citrobacter rodentium 44 sepZ/espZ 3 5 5 5 45 48 49 Lactobacillus rhamnosus 50 + 51 6