Introduction 1999 1991 1993 1999 1999 Arabidopsis 2003 2005 2005 2005a 2006 Os Os Os OsPR10a 1996 OsPR10a OsPR10a 2001 OsPR10a 2006 2006 Magnaporthe grisea OsPR10a OsPR10a OsCHNIII 2001 2004 2006 2006 OsPR10a OsCHNIII Magnaporthe grisea a cis 2005 2006 2006 cis 2000 Arabidopsis PR1 PR1 PR-1 2000 cis 1996 2002 1999 2001 2002 cis 1999 2001 2007 2002 At At Arabidopsis 2003 Arabidopsis 2005 Os Os Os 2005b 2006 2007 Arabidopsis OsPR10a cis OsPR10a Material and methods Plant materials Oryza sativa Xanthomonas oryzae oryzae Xoo O. sativa 2  600 Xoo OsPR10a OsPR10a OsPR10a AAAAAGCAGGCT AGAAAGCTGGGT The underlined sequences match the attB1 and attB2 sites for the Gateway cloning system (Invitrogen, Carlsbad, CA, USA). A 1000 bp amplified PCR product was cloned into pDONR221 to make an entry clone by BP clonase (Invitrogen); successful insertion was confirmed by sequencing. Promoter-LUC constructs OsPR10a OsPR10a PR10a Os r Os OsPR10a AAAAAGCAGGCT AAAAAGCAGGCT AAAAAGCAGGCT AGAAAGCTGGGT Os Os r Site directed mutagenesis OsPR10a OsPR10a Pfu Turbo Dpn E.coli Particle bombardment and transient expression assays Os Os Os 1962 Os Os RT-PCR analysis 16  OsPR10a OsPR10a 5′-GCTACAGGCATCAGTGGTCA-3′ and 5′-GACTCAAACGCCACGAGAAT-3′, OsActin 5′-TCCATCTTGGCATCTCTCAG-3′and 5′-GTACCCGCATCAGGCATCTG-3′. Arabidopsis Os OsPR10a Agrobacterium tumefaciens Arabidopsis Arabidopsis A. tumefaciens Os A. tumefaciens Os Arabidopsis 1  1 2 2 Os Results OsPR10a OsPR10a OsPR10a OsPR10a 1996 2006 1 OsPR10a Xoo 2006 OsPR10a OsPR10a 1 OsPR10a 1 OsPR10a Xoo Fig. 1 OsPR10a Xoo a Xoo b c OsPR10a OsActin  OsPR10a OsPR10a OsPR10a OsPR10a OsPR10a OsPR10a OsPR10a OsPR10a Os 2 Os OsPR10a 2 2 OsPR10a Fig. 2 OsPR10a a OsPR10a b OsPR10a Os Materials and methods Os Os Bars  OsPR10a Arabidopsis  OsPR10a OsPR10a Os Arabidopsis Agrobacterium 3 Arabidopsis OsPR10a Arabidopsis 3 Arabidopsis Os 3 3 3 OsPR10a Arabidopsis Fig. 3 Arabidopsis Os a Os b OsPR10a Os Arabidopsis Agrobacterium Arabidopsis Os Arabidopsis left panel bright-field upper panel middle panel bottom panel  cis OsPR10a cis http://www.dna.affrc.go.jp/cDNA/place 4 cis cis OsPR10a 1 1995 1999 1999 OsPR10a 4 cis 1997 2002 2004 OsPR10a cis 4 OsPR10a 1 cis OsPR10a Fig. 4 cis OsPR10a cis Arrows cis W-box RAV1AAT ASF1 motif WLE1  OsPR10a OsPR10a OsPR10a 5 5 OsPR10a 5 Os cis OsPR10a 5 OsPR10a 4 cis OsPR10a 1 OsPR10a Fig. 5 OsPR10a a OsPR10a cis- bold b OsPR10a Os Materials and methods Bars OsPR10a OsPR10a  Table 1 cis OsPR10 a a b cis  c III −687 to −637 GT1CONSENSUS(1)/GT-1, WLE1 (1)/WRKY a OsPR10a OsPR10a b OsPR10a c cis OsPR10a http://www.dna.affrc.go.jp/cDNA/place OsPR10a 6 1999 OsPR10a OsPR10a OsPR10a OsPR10a Fig. 6 OsPR10a a OsPR10a b  Discussion Arabidopsis; OsPR10a OsPR10a 2006 2006 OsPR10a 1996 OsPR10a Magnaporthe grisea 2001 OsPR10a OsPR10a Xanthomonas oryzae oryzae 2001 2001 1996 OsPR1 1996 OsPR10a OsPR10a OsPR10a OsPR10a 1996 OsPR10a 2001 OsPR10a OsPR10a Arabidopsis OsPR10a OsPR10a 2006 Magnaporthe grisea OsPR10a OsPR10a cis OsPR10a cis 1998 2002 2006 OsPR10a 1997 2002 2004 OsPR10a 1996 OsPR10a cis OsPR10a OsPR10a OsPR10a cis OsPR10a OsPR10a OsPR10a cis OsPR10a 1 2000 PR-1 1999 OsPR10a cis Os Os Arabidopsis 2001 2001 Arabidopsis 2005b Os OsNPR1 OsPR1b Os OsNPR1 OsPR1b OsWRKY12 OsPR10a OsPR10a OsPR10a OsPR10a