Introduction 2003 2005 1998 2000 2002 2001 2004 1996 ys1 65 2006 62 62 2+ 2+ 2+ 1993 1994 1994 1989 1995 2000 3 1987 l 2001 2000 HvNAS1 HvNAAT-A HvNAAT-B HvDMAS1 HvIDS2 HvIDS3 1993 1994 1999 1999 2006 OsNAS1 OsNAS2 OsNAAT1 OsDMAS1 2003 2006 OsNAS3 2003 2003 NASHOR2 NAS 1999 HvNAAT-B IDS2 IDS3 2006 HvNAS1 HvNAAT-A HvNAAT-B IDS2 IDS3 2006 The aims of the present study were to examine if the stimulation of MAs secretion by Zn deficiency is common in graminaceous plants and roles of MAs in Zn homeostasis of rice plants. In this report, we shows that DMA secretion is decreased in rice roots, while the concentration of endogenous DMA is increased in Zn-deficient rice shoots. Materials and methods Plant material and growth conditions Oryza sativa  2001 Measurement of DMA secretion and endogenous levels of DMA and NA 2006 2001 4 + 1987 1989 Northern blot analysis + OsNAS1 OsNAS2 OsNAS3 OsNAAT1 OsDMAS1 OsNAS1 OsNAS2 OsNAS3 OsNAAT1 OsDMAS1  62 1997 62 2001 62 2+ 62 2+ −2  −1 −2  −1 62 62 2001 62 62 62 Results Plant growth, DMA synthesis and gene expression 1 2006 Fig. 1 n  2 P Fig. 2 n  3 OsNAS1 OsNAS2 OsNAS3 OsNAAT1 OsDMAS1 2003 2008 2006 OsNAS3 OsNAAT1 OsNAS1 OsNAS2 OsDMAS1 OsNAS2 OsNAS1 OsNAS2 2 3 Fig. 3 a b l  OsNAS1 OsNAS2 OsNAAT1 OsDMAS1 OsNAS3 OsNAS3 OsNAAT1 OsDMAS1 OsNAS1 OsNAS2 3 2001 4 4 OsNAAT1 3 OsNAAT1 4 Fig. 4 a b n  Contribution of DMA to Zn translocation in Zn-deficient rice 5 1 62 62 62 62 2+ 62 5 62 5 62 2+ 62 5 2+ 62 5 62 62 2+ 62 5 62 62 62 2+ 5 2+ 62 62 1 62 2+ 62 Fig. 5 62 a b c d 62 e 62 d f d  2+ 6 2 62 6 62 62 62 62 2+  6 62 62 2+  3 62 62 62 2+ 62 62 2+ Fig. 6 62 a 62 b c 62  Discussion The concentration of endogenous DMA is increased in Zn-deficient shoots, but DMA secretion from the roots is not increased 2006 2 2006 2 2006 OsNAS1 OsNAS2 OsDMAS1 3 2003 OsNAS1 OsNAS2 2 3  OsNAS3 2003  OsNAS3 2003 OsNAS3 3 OsNAS3 OsNAS1 OsNAS2 4 OsNAAT1 OsNAAT1  4 Zn-DMA is the preferred form for long-distance transport of Zn in Zn-deficient rice 2+ 2006 2+ 5 2 2+ 62 62 62 2+ 5 62 62 62 2+ 6 2006 Electronic supplementary material  (EPS 348 kb) (EPS 362 kb) (EPS 353 kb) (MOV 2400 kb) (MOV 2257 kb)