Introduction 1995 1997 1987 1996 1993 1994 Arabidopsis thaliana 2001 Phaseolus lunatus 1994 1996 Nicotiana tabacum N. attenuata Zea mays Gossypium hirsutum 1998 1999 2004 2000 Cucumis sativus 1999 2000 2000 2004 Artemisia annua 2002 Pieris rapae AtTPS10 AtTPS03 2001 LjEβOS Lotus japonicus 2004b 2002 2005 1995 2004a 2005 2002 2000 LeMTS1 LeMTS2 LeMTS1 Results LeMTS1 LeMTS2 1 LeMTS1 LeMTS2 LeMTS1 LeMTS2 1 1998 2+ 2+ 1996 1997 2+ 2+ 2001 2002 2000 Fig. 1 L. esculentum L. esculentum 8 1998 1996 1997 3 3 2001 2002  1 LeMTS1 and LeMTS2 belong to the TPSb subfamily of terpene synthases 1 TPSb 2 1997 Antirrhinum majus 2003 Fig. 2 1997 1996 2003  Terpene synthase activity of recombinant LeMTS1 and LeMTS2 Escherichia coli 8 1 1999 1998 3 2001 E. coli 3 2002 1999 2002 3 Fig. 3 A B E  R E 3 LeMTS1 LeMTS2 LeMTS1 LeMTS2 4 LeMTS1 LeMTS2 LeMTS2 Fig. 4 LeMTS1 LeMTS2 A LeMTS1 LeMTS2 B RCE1  1992 2004 2005 1999 2004 2001 1985 LeMTS1 LeMTS2 LeMTS1 LeMTS2 4 LeMTS1 LeMTS2 LeMTS1 LeMTS2 LeMTS LeMTS1 LeMTS2 5 WIPI-II PRP6 2004 5 LeMTS1 LeMTS2 5 PRP6 LeMTS1 LeMTS2 LeMTS1 WIPI-II 5 2004 2004 defenseless1 def-1 1996 LeMTS1 5 Fig. 5 LeMTS1 A LeMTS1 LeMTS2 WIPI-II 1985 PRP6 1992 RCE1 B LeMTS1 WIPI-II RCE1  LeMTS1 def-1 1996 NahG 2000 def-1 LeMTS1 6 NahG LeMTS1 6 LeMTS1 Fig. 6 LeMTS1 JA A LeMTS1 wt def-1 B LeMTS1 NahG RCE1  LeMTS1 4 7 Fig. 7 LeMTS1 WIPI-II RCE1  LeMTS1 4 LeMTS1 8 E Fig. 8 Linalool accumulation in trichomes. Relative levels of linalool, a selection of other terpenes and methyl salicylate in extracts of petioles and stems before and after removal of trichomes compared to total leaf and root extracts. Equal amounts of the different tissues were used (freshweight, 0.5 g), and trichome material was corrected for freshweight of the original petiole or stem. Averages of two measurements and min/max are shown  LeMTS1 LeMTS1 5 9 Fig. 9 A B  LeMTS1 LeMTS1 LeMTS1 9 Discussion 2004 LeMTS1 LeMTS2 LeMTS1 Identification of two tomato monoterpene synthases R E 2 LeMTS1 5 9 3S E S 2004 2003 2006 Litospermum erythrorizon 2000 1995 1999 LeMTS1 5 9 1 Citrus limon 2 2004 2000 2006 Salvia officinalis Mentha citrata 2002 1 1 LeMTS1 LeMTS1 LeMTS1 5 9 LeMTS1 5 2004 LeMTS1 9 LeMTS1 2004 2003 2001 2003 2006 2004 2001 1999 LeMTS1 LeMTS2 LeMTS2 4 8 2004 2004 LeMTS2 4 1987 2004 LeMTS2 8 LeMTS2 4 LeMTS1 2006 2004 2000 LeMTS1 7 WIPI-II WIPI-II WIPI-II Solanum americanum 2006 WIPI-II WIPI-II 2005 2003 Datura wrightii 2006 Nicotiana repanda 2000 3 2 2 2005 LeMTS1 Materials and methods LeMTS1 LeMTS2 Lycopersicon esculentum Tetranychus urticae 2004 - LeMTS 1991 2001 LeMTS1 LeMTS2 2004 2000 E. coli LeMTS1 LeMTS2 1 LeMTS1 LeMTS2 Pfu LeMTS1 CCATGG CTCGAG LeMTS2 CCATGG CTCGAG LeMTS E. coli 600 d 2 2 g 2001 Plant treatments, plant headspace sampling, trichome isolation, and measurement of extracts 2 2004 2 2 2 2004 Gene expression analysis by RT-PCR and real-time Q-RT-PCR RCE1 RCE1 RCE1 RCE1 WIPI-II PRP6 LeMTS1 LeMTS2 LeRCE1 LeWIPI2 LeMTS1 RCE1 Generation of transgenic tomato plants LeMTS1 , 2002 Agrobacterium tumefaciens 2000 1993 LeMTS1 LeMTS1