Introduction 4 4 1 2 4 Morinda citrifolia in vitro 5 4 4 4 4 Materials and Methods Materials Carbon tetrachloride was purchased from Sigma Chemical Co., St. Louis, MO, USA. Noni juice was made by Morinda Holdings Inc. (Provo, UT, USA) from Noni fruit originating in Tahiti. The placebo was prepared using the same procedures and ingredients for making Tahitian Noni® Juice (TNJ), but without the Noni component. Animals ad libitum Experiment 1 Animal Treatment 4 4 LM Examination 2 6 ALT and AST Assay Serum was separated from clotted blood by centrifugation at 1,600 rpm for 10 min at 4 °C. Serum ALT and AST activities were measured using an automatic biochemical analyzer calibrated with quality control standards (E.I. du Pont de Nemours) in a clinical laboratory (Family Medicine Laboratory, UIC College of Medicine at Rockford). Experiment 2 Animal Treatments 4 4 LM Examination Liver samples for experiment 2 were prepared and observed for light microscopy as described in experiment 1. Statistical Analysis T 7 P P Results and Discussion 1 4 4 1 1 4 4 1 4 4 P 4 P Fig. 1 d 4 4 a b 4 4 arrow arrowhead c) d 4 b d  4 2 4 4 2 4 2 Fig. 2 4 a 4 b 4 arrow c 4 d 4 e 4 f 4 arrow arrowhead  4 3 3 3 4 3 Fig. 3 4 a 4 b Arrows arrowheads  Noni Juice Suppresses ALT and AST Activities 1 4 P P 4 Table 1 4 Treatment ALT (IU/L) AST (IU/L) Control ** ** Placebo (20%) ** ** Noni juice (20%) ** ** 4 a 136 ± 41 1135 ± 466 4 ** * a 4 n T * P ** P 4 4 4 4 8 9 10 Conclusions 4