Introduction 2004 1993 1996 1995 1996 1994 2004 2004 1995 2003 2004 1990 1987 1994 1999 1981 1999 1999 2002 1994 1997 1987 1999 1991 1992 Amphidinium carterae 1996 2005 2005 1999a 2000a 2000b 2001 2004 2003 2002 2002 1996 a a 1 2 a a 1 1999 2000 2000 2001 2000 2003 2002 2004 1996 Fig. 1 Structure of the pigments in a monomeric subunit of the MFPCP complex. The coordinates were taken from Protein Data Bank with 1PPR code. Also shown are the structures of peridinin and the chlorophylls used in this study  2005 2005 Escherichia coli a a a a b b d d a a a a 2005 Materials and methods Sample preparation a b d a a 1 2005 a b d a Acaryochloris marina Rhodospirillum rubrum A. marina d a R. rubrum a a a a R. rubrum 18 a b −1 d a −1 a a −1 Spectroscopy 2004 Results Absorption spectroscopy a b d a a 2 a b d a a y b a a d a y d −1 −1 a y 2 a a Fig. 2 a b d a a a  2004 3 y 3 a b d a a a b a d a Fig. 3 y dashed line a 2004  4 y a b a 4 d a 4 Fig. 4 y A a B b C d D a a E a  Spectral reconstruction 2004 5 5 a b 5 1 d a a 1 Fig. 5 Reconstruction of the 10K absorption spectra of the reconstituted PCP complexes in the 375–600nm region. The analysis was carried out by linearly combining individual 10 K absorption spectra of peridinin and Chl taken in 2-MTHF solvent as described in the text  Table 1 y PCP complex Peridinin Chl Reference  Soret y 1 2 3 4 5 6 7 8 1 2 1 2 MFPCP 470 485 522 522 526 526 544 544 434 439 665 667 2004 HSPCP na 489 533 533 533 533 533 na 436 441 667 676 2004 a 472 ± 2 486 ± 2 510 ± 1 510 ± 1 529 ± 1 529 ± 1 548 ± 1 548 ± 1 434 ± 1 440 ± 1 667 668  b 470 ± 3 485 ± 2 508 ± 1 508 ± 1 527 ± 2 527 ± 2 546 ± 2 546 ± 2 458 ± 1 461 ± 1 644 647  d 474 ± 2 490 ± 2 513 ± 1 513 ± 1 530 ± 1 530 ± 1 546 ± 1 546 ± 1 456 ± 2 456 ± 2 697 697  a 467 ± 2 482 ± 2 511 ± 2 511 ± 2 530 ± 2 530 ± 2 543 ± 1 543 ± 1 446 ± 1 446 ± 1 683 683  a 472 ± 2 486 ± 2 507 ± 2 507 ± 2 531 ± 1 531 ± 1 545 ± 1 545 ± 1 365 ± 1 365 ± 1 782 782  The numbers are in nm units and the uncertainties represent the range of values that give an acceptable fit to the data MFPCP HSPCP aChl a a na Fluorescence spectroscopy 6 a b d a a a Fig. 6 a b d a a a λ max  Fluorescence excitation spectroscopy 7 T T a b d a a Fig. 7 A a B b C d D a a E a T y  Discussion Low temperature absorption spectra 2 2005 a 2 2004 2 5 2004 2 y d −1 −1 1997 1997 y 1990 −1 d d R1 1 d R1 y 3 y a 4 a 3 a b 4 d a y d a a b 5 a b a b y 8 8 y a b a b d a a Fig. 8 A view of the structure of the MFPCP complex focusing on the Ring I of Chl 601. Per 612, Per 614 and Leu 204 are in close proximity to the Ring I of Chl 601. The coordinates of the structure were taken from Protein Data Bank with 1PPR code  a y 2 4 a Prosthecochloris aestuarii 1991 2002 1978 y a 2002 1979 1986 a 1979 a 1996 y 4 5 d a y a b a d a 3 4 y 1990 R1 R2 1 R1 d a b y a R1 a 2002 2002 a R1 Energy transfer efficiencies 0 2 2 1 0 1996 2001 2003 1 1999 2001 2003 1999 2003 1 2004 1 2003 2003 1 1 2 1 9 ε 1998 1 \documentclass[12pt]{minimal}
\usepackage{amsmath}
\usepackage{wasysym} 
\usepackage{amsfonts} 
\usepackage{amssymb} 
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}
$$ \epsilon \, = \,\frac{{k_{{ET2}} }} {{k_{{ET2}} \, + \,k_{{IC2}} }}\, + \,{\left[ {\frac{{k_{{IC2}} }} {{k_{{ET2}} \, + \,k_{{IC2}} }}} \right]}{\left[ {\frac{{k_{{ET1}} }} {{k_{{ET1}} \, + \,k_{{IC1}} }}} \right]} $$\end{document} k ET2 k ET1 2 1 k IC2 2 1 k IC1 1 0 2005 2 2 1 k IC2 −1 τ IC2 2004 k IC1 −1 τ IC1 2003 7 2 Fig. 9 k ET1 k ET2 solid lines 2 1 k IC2 k IC1 dashed lines 2 1 1 0  Table 2 Rate constants and energy transfer efficiencies obtained from the ultrafast, time-resolved optical spectroscopic experiments on the PCP complexes reconstituted with various Chls PCP complex k ET2 −1 k IC2 −1 k ET1 −1 k IC1 −1 ε ε a 10 ± 2.5 15 ± 1.5 0.34 ± 0.034 0.063 94 ± 2 91 ± 7 b 10 ± 2.5 15 ± 1.5 0.17 ± 0.017 0.063 92 ± 2 84 ± 14 d 13 ± 3.0 15 ± 1.5 0.59 ± 0.059 0.063 96 ± 3 95 ± 2 a 11 ± 2.8 15 ± 1.5 0.45 ± 0.045 0.063 99 ± 1 93 ± 4 a 10 ± 2.5 15 ± 1.5 2.22 ± 0.22 0.063 ∼100 98 ± 2 k ET2 k ET1 2 1 2005 k IC2 k IC1 2 1 1 0 k IC2 2004 k IC1 2002 k IC2 k IC1 ε ε ε a a a b d a a