[7] [8] [10,15] [12,14] [1] [7] [2] [11,13] [5] −/− [6] in vitro waf l t * p For sub-cellular localisation experiments HEK293a cells were transfected with human 2N4R tau independently or in combination with p53 as described above. The following day the cells were fixed in ice-cold methanol and stained according to standard protocols. Cells were incubated with rabbit anti-total tau (1:500) or mouse anti-p53 (1:250) before being incubated with the appropriate fluorescent secondary antibody (1:200). Nuclei were counter-stained with Hoescht 33342. Immunofluorescence was captured using a Zeiss LSM510 meta-confocal microscope. All experiments were performed in triplicate; therefore, figures shown are representative of a single experiment. waf [5,11,13] Fig. 1 Fig. 1 Fig. 2 Fig. 2 [4] Fig. 2 Fig. 2 Fig. 2 Fig. 2 Fig. 2 waf Fig. 2 [9] [3] [13]