Introduction [3] [4,5] in vitro [6,7] CBS [8] [9] [10] Escherichia coli Saccharomyces cerevisiae [11,12] [9] E. coli S. cerevisiae [13,14] [15] [16,17] cis trans Materials and methods Yeast strains and growth assays [18,19] [20] Fig. 1 600 600 Immunoblot and CBS enzyme analysis [16] [15] [11] E. coli [21] [22] Fig. 4 In vitro transcription/translation www.promega.com [21] In vitro Results DMSO can rescue growth defect of I278T expressing S. cerevisiae Fig. 1 600 600 600 600 Chemical chaperone rescue of I278T and other missense mutations [23] N Fig. 2 [19] Fig. 2 Chaperone mixtures Fig. 3 600 Predicted change in solvent accessible surface area predicts chemical chaperone rescue [24] Table 1 Chemical chaperones cause enhancement of specific activity and tetramer formation of mutant CBS S. cerevisiae Fig. 4 Fig. 4 Chaperone effect requires protein folding and is not a phenomena of S. cerevisiae E. coli [21] Fig. 5 in vitro Fig. 5 E. coli Discussion [25] [26] G [27] A I A I I A I A [16,17] [28–31] 50 http://physchem.ox.ac.uk/MSDS [32] [33] in vivo