Introduction Saccharomyces cerevisiae 1 2,3 bona fide In vitro 4 5 6 in vivo 7–9 10 11 12 13 14–16 via 10,17 in vitro 18–20 via K m via K cat K m in trans in cis Results Generation of modified chromatin Xenopus laevis 21,22 Figure 1 20 Supplementary Data, Figure 1 19,23 in vivo Escherichia coli Figure 1 via Figure 1 Figure 1 24 Lysine acetylation can alter intrinsic nucleosome mobility 25 26 Figure 2 Figure 2 27 Supplementary Data, Figure 2 Supplementary Data, Figures 2, 3, 4 Figure 2 Figure 2 Figure 2 The RSC complex preferentially repositions tetra-acetylated H3 but not H4 nucleosomes 28 S. cerevisiae 29,30 Figure 3 Figure 3 Figure 3 Figure 3 Figure 3 Supplementary Data, Figure 3 Figure 3 K m K cat via 31 K m V max Figure 4 K m K cat Figure 4 Supplementary Data, Figure 5 K m Figure 4 K m Figure 4 32 Figure 4 K cat K m K cat 33–36 19,37 Figure 5 Figure 5 37 K cat K m Figure 5 Supplementary Data, Figure 6 The yeast Chd1 enzyme requires the H4 tail for efficient chromatin remodelling 1 Figure 6 Figure 6 versus Figure 6 K m Supplementary Data, Figure 6 Figure 6 in trans in vitro 38,39 32 Figure 7 Figure 7 Figure 7 Figure 7 K m Figure 4 Figure 3 Figure 7 Figure 4 in trans Supplementary Data, Figure 7 Discussion Histone acetylation alters the intrinsic dynamic properties of nucleosomes 40 41–46 via 47 48–50 19,51–53 48,49,54 55 24 Figure 2 Figure 2 53 27 in vivo K m K cat 1 Figure 4 12,56 32 12,56 Figure 4 57,58 46 K cat Figure 5 34 Figure 6 S. cerevisiae 59 60 in vitro 61 62 Drosophila 63 1 in trans in trans 64–66 67,68 in trans Figure 7 69 Figure 7 in cis Figure 3 Figure 4 Supplementary Data, Figure 7 70,71 72 42,46 73 74 75,76 Experimental Procedures Purification of remodelling enzymes A 600 d 2 77 et al. 61 Supplementary Data, Figure 8 Native peptide ligation 21,22 Nucleosome reconstitution Xenopus laevis 78 79 80 81 32 Nucleosome remodelling y x n+x 2 2 ATPase assay 31 2 31 Octamer transfer and dimer exchange 72 81 1 Supplementary data Supplementary material  A Supplementary Data doi:10.1016/j.jmb.2007.09.059