Introduction 1–4 5–7 NCAM1 8 9–12 13 14,15 cis trans 16 17 cis 18–22 23–26 27 Fig. 1 20,28,29 1 30 2 28 1 2 28,31 1 2 1 2 1 2 Results Crystal structure of the NCAM FN3 tandem 1 2 Table 1 1 2 Fig. 2 1 2 33 34 1 31 1 1 31 α 2 2 28 α 1 2 1 2 2 1 2 Fig. 2 Fig. 2 30 1 2 Fig. 2 1 2 2 2 Fig. 2 35,36 1 2 1 2 Structure of the M610R mutant 1 2 Fig. 3 1 2 1 2 Fig. 3 1 1 1 2 2 1 2 38–40 33 Drosophila 37 Location of putative FGFR1 binding site 1 2 et al. 2 28 1 30 30 Fig. 4 1 1 2 Surface plasmon resonance analysis of the NCAM–FGFR1 interaction 1 2 et al. 28 Fig. 5 Fig. 6 1 2 Fig. 6 1 2 Fig. 6 1 2 1 2 Discussion NCAM ectodomain structure NCAM splice variants 1 2 R 9,10,12 1 in vitro 42,43 44 1 2 Interaction of NCAM with FGF 1 2 1,22,30 1 2 28,30 1 2 Fig. 4 1 2 1 2 31 45 1 2 28,29 20 1 2 Fig. 5 1 2 Fig. 6 et al. 29 et al. 46 1 2 1 2 Experimental Procedures Expression vectors P13591 P13591 12 P11362 47 6 1 2 Protein expression and purification 2 4 Crystallisation and structure determination SPR experiments N Protein Data Bank accession codes 1 2 2vkw 2vkx