Introduction 2006 1994 2002 2003 2001 2006 2004 NOS3 1999 2003 NOS3 2004 NOS3 NOS3 NOS3 MTHFR Materials and methods Study population 2006 n n 1995 1999 For the present study, DNA of 102 spina bifida patients, 116 mothers, 101 fathers, 265 control women and 211 pediatric controls was available for genotyping. Biochemical determinations 1995 Plasma folate concentration was determined using the Dualcount Solid Phase Boil Radio assay (Diagnostic Product Corporation, Los Angeles, CA). PCR amplification and genotype determination NOS3 1999 Mbo Statistical analyses Case-control study Odds ratios (ORs) and their 95% confidence intervals (95%CI) were calculated by means of logistic regression analysis. NOS3 Deviation from Hardy–Weinberg equilibrium was calculated to assess the accuracy of the genotyping assay. 2003 P Transmission disequilibrium test NOS3 1993 2003 Results Case-control study NOS3 P 1 NOS3 NOS3 NOS3 NOS3 NOS3 NOS3 NOS3 NOS3 NOS3 Table 1 NOS3 NOS3 Mothers (%) Female controls (%) OR (95%CI) GG 49 (42.6) 130 (50.2) a GT 53 (46.1) 106 (40.9) 1.33 (0.83–2.11) TT 13 (11.3) 23 (8.9) 1.50 (0.71–3.19) a NOS3 NOS3 P NOS3 NOS3 2 NOS3 NOS3 NOS3 NOS3 NOS3 Table 2 NOS3 NOS3 SB patients (%) Pediatric controls (%) OR (95%CI) GG 39 (41.1) 111 (53.6) a GT 46 (48.4) 80 (38.6) 1.64 (0.98–2.74) TT 10 (10.5) 16 (7.7) 1.78 (0.75–4.25) a Transmission disequilibrium test NOS3 Χ 2 P Gene-gene interaction NOS3 MTHFR MTHFR MTHFR MTHFR MTHFR NOS3 NOS3 MTHFR 3 Table 3 NOS3 MTHFR NOS3 MTHFR Mothers (%) Female controls (%) OR (95%CI) GG CC/CT 42 (38.2) 114 (44.0) a GG TT 6 (5.5) 16 (6.2) 1.02 (0.37–2.77) GT/TT CC/CT 52 (47.3) 123 (47.5) 1.15 (0.71–1.85) GT/TT TT 10 (9.1) 6 (2.3) 4.52 (1.55–13.22) a NOS3 MTHFR Gene-metabolite interactions MTHFR NOS3 NOS3 NOS3 NOS3 4 Table 4 NOS3 NOS3 a Mothers (%) Female controls (%) OR (95%CI) GG <12.56 42 (36.8) 100 (38.6) b GG ≥12.56 7 (6.1) 30 (11.6) 0.66 (0.26–1.65) GT/TT <12.56 47 (41.2) 108 (41.7) 1.17 (0.69–1.96) GT/TT ≥12.56 18 (15.8) 21 (8.1) 3.38 (1.46–7.84) a b Discussion NOS3 NOS3 MTHFR NOS3 2004 NOS3 NOS3 2004 NOS3 NOS3 NOS3 NOS3 2004 NOS3 MTHFR NOS3 2001 2000 2001 2004 NOS3 S 1993 2004 S S NOS3 NOS3 NOS3