Introduction 2002 2006 2002 1 2000 2002 2003 1990 1994 1994 1 2001 Fig. 1 a b c Lymnaea stagnalis Ls magenta black 2007 Ls above bold yellow cyan top  2003 2006 2006 2004 1999 2002 2001 2007 2007 Electrophorus electricus 1980 2000 2005 2007 1 1 2002 2006 2007 2003 Xenopus laevis 1 Drosophila 2004 Xenopus Drosophila 2006 Drosophila 2006 2006 2004 2007a 2001 2005 2005 2004 2005 2002 2003 2006 1 Lymnaea stagnalis Ls 2001 2001 Ls Aplysia californica Ac 2005 Bulinus truncatus Bt 2005 Ls Ac- 2001 2005 2004 2005 2007 2005 2007 2006 2006 Ls- Materials and methods Materials Lymnaea stagnalis N N′ N′′ 2000 2000 Protein preparation Ls L. stagnalis Pst Ls Pichia pastris Pichia N 3 Evaluation of equilibrium binding of neonicotinoids Ls L 3 Ls \documentclass[12pt]{minimal}
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$$ \frac{{\Delta F}} {{F_{0} }} = \frac{{\Delta F_{{\max }} }} {{F_{0} }} \cdot \frac{{K_{{\text{d}}} + [{\text{X}}] + [{\text{Protein}}] - {\sqrt {{\left( {K_{{\text{d}}} + [{\text{X}}] + [{\text{Protein}}]} \right)}^{2} - 4 \cdot [{\text{X}}] \cdot [{\text{Protein}}]} }}} {{2 \cdot [{\text{Protein}}]}}, $$\end{document} F F max F 0 Ls Crystallization and X-ray data collection Ls Ls 2007 2001 1992 1997 1 Table 1 Ls  IMI CTD Br-IMI Br-CTD a Data collection b BL44XU BL44B2 BL44B2 BL44B2 BL44B2  Wavelength (Å)  0.900 0.919 0.919 0.919 1.75  Space group P 5 P 5 P 5 P 5 P 5 a c 75.0, 351.0 74.6, 351.0 74.8, 350.9 74.5, 351.2 74.6, 351.3  Resolution (Å) 15.8–2.56 50.0–2.70 50.0–2.70 50.0–2.90 50.0–2.90 R sym c, d 8.0 (41.3) 8.5 (38.9) 9.7 (34.1) 11.8 (34.1) 8.7 (39.5) I σ c 8.1 (1.7) 20.7 (4.0) 47.0 (10.7) 45.5 (10.9) 60.8 (9.32) c 94.8 (96.7) 100 (100) 100 (100) 100 (100) 99.5 (96.2) c 2.6 (2.6) 5.7 (5.7) 23.0 (23.3) 22.5 (23.2) 21.6 (20.5) Refinement  Resolution (Å) 15.8–2.58 50.0–2.70     No. of reflections 33031 30121    R R free e 20.3/27.7 20.2/27.0    f 0.008/1.3 0.008/1.3     Average B factor 41.8 41.6    g 2ZJU 2ZJV    See text for abbreviations of the neonicotinoid names a b c d R sym I I I I I e R F o F c F o R free R f g Structure determination and refinement 2007 Ls 2004 1998 2004 1 1999 1997 Results and discussion Structure analysis Ls 3 Ls 1 Ls 2 2001 2004 2005 2005 2006 Ls 2004 2 2000 2001 2005 2 Ls Ls 2 Fig. 2 Ls- Ls- a b Ls- green blue red pink blue c Ls- red Ls- blue light brown 2004  Interactions with aromatic rings of insecticides Ls 3 2008 Ls Ls Ls 2004 Ac 2005 Fig. 3 F o –F c blue a b Ls- purple yellow c d Ls- purple blue red yellow orange broken lines  3 3 2004 3 2003 1 Interactions with guanidine and related moieties Ls 2004 Ac 2005 2005 2005 2005 Ls 4 1 4 2 2 K d n K d n Ls Fig. 4 Lymnaea stagnalis Ls a Ls b Ls green cyan a b Orange broken lines purple blue red yellow  2007b Ls 4 2003 2005 2006 2004 Interactions with loop D and selectivity of neonicotinoids Ls 1 2002 L Ls 4 1 Ls Ls 5 Fig. 5 Ls- green cyan purple blue red yellow Orange broken lines  Role of loops C and F in their interactions with neonicotinoids Drosophila 2004 Ls 1 2007 Ls 5 Ls 1 1 Ls