Introduction Mhc 2005 1989 2004 2005 2005 2006 1995 1998 1999 2001 2002 2004 2004 2005 2005 2006 2006 MhcMamu HLA-DP DQ DR 1999 Mamu-DRB 2000 DRB 2004 Mamu-E 1998 -F 1993 -G 1996a 1996 -AG 1997 B Mamu-I 2000 HLA-C HLA-A -B 1996b Mamu-A -B 2004 2004 Mamu-A B 2005 Mhc Mhc Mhc 1998 1998 Mhc 2006 2006 Mafa-A 2004 2005 Mafa-A Mhc A Materials and methods Animals and cell lines 1999 2005 2006 cDNA cloning and sequencing A Mamu-A 2005 2005 Locus-specific PCR reactions Mamu Mafa-A4*14 Phylogenetic analysis and nomenclature The sequences were analyzed with the Sequence Navigator Software version 1.0.1 (Applied Biosystems), and alleles are based on at least three clones with identical full-length sequences. To define loci and lineages, alignments of the sequences were made using the MacVector™ version 8.1.1 (Oxford Molecular Group), followed by manual adjustments. Phylogenetic analyses on the full-length (1,065 bp) sequences were also performed with the MacVector software. Neighbor-joining trees were constructed with the Kimura 2 parameter method. Bootstrap analyses were performed based on 1,000 replications. Mamu-A Mafa-A 1 Mamu-A Mafa-A 1990 2003 2006 Mamu-A1*0101 Mhc A1 Table 1 Mamu-A Mafa-A Allele Accession Animals Allele Accession Animals Mamu-A1 Mafa-A1 A1*0102 AM295885 EKK A1*0101 AM295828 Yabaa A1*0103 AM295886 Ri056 A1*0301 AM295829 Hoeba A1*030102 AM295887 Ri185 A1*0302 AM295830 77 A1*0302 AM295888 r300 A1*1002 AM295831 k71 A1*0303 AM295889 8822 A1*1003 AM295832 Trufo A1*0304 AM295890 Ri301 A1*1702 AM295833 Bufo A1*0305 AM295891 Ri037 A1*1901 AM295834 77 A1*040102 AM295892 Ri165 A1*2202 AM295835 k94 A1*0402 AM295893 Ri366 A1*3101 AM295836 Gayo A1*1001 AM295894 Ri002 A1*3102 AM295823 Bilboa A1*1002 AM295895 Ri142 A1*3201 AM295824 80 A1*1102 AM295896 Ri136 A1*3801 AM295825 k66 A1*1701 AM295897 Ri145 A1*4002 AM295826 Pedro A1*1801 AM295898 Ri011 A1*4102 AM295827 k65 A1*1802 AM295899 Ri018 A1*4302 AM295837 k65 A1*1803 AM295900 Ri260 A1*5201 AM295838 88 A1*1902 AM295901 Ri081 A1*5301 AM295839 k390 A1*1903 AM295902 BB10 A1*5501 AM295840 k127 A1*1904 AM295903 Ri197 A1*5601 AM295841 k766 A1*2201 AM295904 Ri009 A1*5701 AM295842 k2, A1*2502 AM295905 Ri088 A1*5801 AM295843 Rastafa A1*2602 AM295906 8765 A1*5901 AM295844 Roza A1*2802 AM295907 Ri082 A1*6001 AM295845 Vivaa A1*3201 AM295908 Ri094 A1*6101 AM295846 k135 A1*3301 AM295909 Ri253 A1*6201 AM295847 81 A1*4001 AM295910 Ri165 A1*6301 AM295848 Hippo A1*4101 AM295911 Ri284 A1*6401 AM295849 Bufo A1*4701 AM295912 Ri189 A1*6402 AM295850 k95 A1*4801 AM295913 8822 A1*650101 AM295851 81 A1*4901 AM295914 Ri088 A1*650102 AM295852 k390 A1*5001 AM295915 4053 A1*6601 AM295853 k73 A1*5101 AM295916 Ri281 A1*6602 AM295854 Milva A1*5201 AM295917 Ri136 A1*6701 AM295855 83 A1*5301 AM295918 Ri056 A1*6801 AM295856 Juanita A1*5401 AM295919 Ri260 A1*6901 AM295857 Zazaa A1*5402 AM295920 Ri011 A1*7001 AM295858 Friko A1*5601 AM295921 Ri146 A1*7101 AM295859 Zola A1*5602 AM295922 Ri228 A1*7201 AM295860 Canada A1*5701 AM295923 Ri006    A1*5801 AM295924 Ri026    Mamu-A2 Mafa-A2 A2*050301 AM295925 Ri056 A2*0501 AM295861 Clint A2*050302 AM295930 Ri226 A2*0502 AM295862 84 A2*050402 AM295926 Ri009 A2*0503 AM295863 k95 A2*0513 AM295927 Ri159 A2*0504 AM295864 Clint A2*0514 AM295928 Ri095 A2*0505 AM295865 Bufo A2*0515 AM295929 Ri009 A2*0506 AM295866 Trufo A2*0517 AM295931 Ri284 A2*0507 AM295867 80, A2*0518 AM295932 Ri094 A2*0508 AM295868 Zebra A2*0519 AM295933 Ri137 A2*0509 AM295869 81 A2*0520 AM295934 Ri197 A2*0510 AM295870 Milva A2*0521 AM295935 Ri126 A2*0511 AM295871 Hippo A2*0522 AM295936 Ri026 A2*051201 AM295872 k73 A2*0523 AM295937 Ri028 A2*051202 AM295876 Dojo A2*0524 AM295938 Ri301 A2*0513 AM295873 81 A2*0525 AM295939 Ri137 A2*0514 AM295874 k95 A2*0526 AM295940 Ri002 A2*0515 AM295875 Juanita A2*2402 AM295941 BB67 A2*0516 AM295878 Jawa A2*2403 AM295942 Ri366 A2*0517 AM295877 Laba Mamu-A3 Mafa-A3 A3*1307 AM295943 Ri136 A3*1301 AM295879 k2, Mamu-A4 Mafa-A4 A4*1404 AM295944 Ri137 A4*1401 AM295880 77    A4*1402 AM295881 Bufo Mamu-A5 Mafa-A5 A5*300101 AM295945 BB67 A5*3001 AM295882 Gayo A5*300102 AM295946 Ri081 A5*3002 AM295883 k766 A5*3002 AM295948 Ri088    A5*3003 AM295947 Ri366    Mamu-A6 Mafa-A6 A6*0101 AM295949 Ri145 A6*0101 AM295884 k73 Mamu-A7    A7*0101 AM295950 Ri082    A7*0102 AM295951 Ri315    A7*0103 AM295952 Ri009    The Chinese rhesus macaques are indicated by Ri numbers. Nine alleles found in rhesus macaques of mixed breeding are also reported. Results and discussion Mamu-A Mamu-A 2005 Mamu-A Mamu-A1 -A2 -A3 -A4 1 Mamu-A1 Mamu-A Mamu-A1 1999 2000 2005 2004 2004 -A1/-A2 -A1/-A4 1 Fig. 1 Mamu-A polymorphism transcription Mamu-A 2004 2004  Mamu-A 2 5 Mamu-A1 Mamu-A1 2 -A5 -A6 -A7 -A2 -A3 -A4 Mamu-A5 -A6 and -A7 1990 2003 2006 A5 A6 A1 Fig. 2 Mamu-A Mafa-A -A2 Mamu-A Mafa-A Asterisk Mamu-A5 Double asterisks Mamu-A6 Triple asterisks Mamu-A7  Mamu-A1 Mamu-A1 Mamu-A1*26 Mamu-A1*03 -A1*04 Mamu-A1 Mamu-A1 1988 2004 Mamu-A2 1 Mamu-A2 Mamu-A2 Mamu-A3*1307 Mamu-A1*1102. Mamu-A3 Mamu-A4*1404 Mamu-A4 1 Mamu-A4 Mamu-A5 -A6 -A7 Mamu-A 2 Mamu-A5, -A6 -A7 Table 2 Mamu-A   Mamu-A1 Mamu-A2 Mamu-A3 Number 1 *1801 *1516  3 *2201 *0515  6 *5201 *0513  3 2 *1102  *1307 5 Mafa-A Mafa-A1 Mamu-A2 -A3 -A4 -A5 -A6 Mafa-A1 2004 2005 -A1*3101 -A1*3201 -A1*3801 -A5*3001 A*310101 A*320101 A*380101 A*300101. Mafa-A2 1 A2 Macaca nemestrina 2006 2007 Mane-A Mafa-A3 Mafa-A4 Mafa-A4 2 Mafa-A4 Mafa-A5 -A6 2 Mafa-A1 3 3 4 1 Mafa-A5*3001 Mafa-A1*6001 -A1*7201 Mafa-A6 Fig. 3 Mafa-A shading question mark  Table 3 Mafa-A   Mafa-A1 Mafa-A2 Mafa-A3 Mafa-A4 Mafa-A5 Number 1 A1*4002     6 A1*5801     4 A1*7001     3 2 A1*0101 A2*0514    4 A1*1003 A2*0506    10 A1*1901 A2*0513    3 A1*3101 A2*0504    4 A1*5901 A2*0508    12 A1*6301 A2*0501    7 A1*6301 A2*0511    3 A1*6401 A2*0505    9 A1*6801 A2*0515    3 A1*6601 A2*051201    3 A1*6602 A2*0510    10 A1*6801 A2*0515    3 3 A1*5701  A3*1301   2 4 A1*1702   A4*1402  11 A1*7101   A4*14  6 5 A1*650102 A2*05  A4*14  3 6  A2*051202  A4*14  9 7 A1*6001    A5*3001 2 A1*7201    A5*3001 3 Mafa-A1 Fig. 4 Mafa-A 1 Mafa-A1 Mafa-A1  -A1 -A2 Mamu-A Mafa-A -A1 -A2 4 2002 A3 A7 Table 4 Identical Mhc class I sequences detected in the two species of macaque Macaca mulatta Macaca fascicularis Mamu-A1*0103 Mafa-A*260101 a Mamu-A1*4001 Mafa-A*400101 a Mamu-A1*1001 Mafa-A1*1002 Mamu-A*21 b Mafa-A1*0302 Mamu-A1*5301 Mafa-A1*5301 Mamu-A2*0519 Mafa-A2*051202 a 2005 b 2005 Mhc Mamu-A Mafa-A Mhc-DR -DQ -DP 2006 2006 Mafa-DPB1 -DQA1 -DQB1 Mafa-DRB Mhc Mhc Mhc Mhc 2003 Mhc Mhc Mhc 1992 1992 Mhc Electronic supplementary material Below is the link to the electronic supplementary material. 
 Table 5 Mamu-A A*05