Introduction 2005 2000 1997 1998 1998 1998 2001 2003 2003 1998 1987 2003 2000 trans trans 2003 1 1998 2001 2003 2001 2003 2003 2003 2005 Fig. 1 arrows a b c d e Arrows  1986 trans- trans 1989 1985 1995 trans 1995 1997 1997 1998 2006 2006 trans trans 1999 2002 trans trans 1985 trans trans 1999 trans trans trans 1999 2002 trans trans 1995 1995 trans 2003 1983 1989 trans 2002 trans 2004 2004 trans 1986 1992 1998 1988 1997 Formation of PGC precursors at the TGN There are two key aspects to the formation of the PGC precursors: their morphogenesis at the TGN; and the segregation between different types of cargo proteins that are targeted for different destinations, as well as between the Golgi-resident proteins, such as the Golgi enzymes. 2002 2002 2003 1995 2003 2003 2001 2006 trans 2004 2005 trans 1999 2002 trans 1996 trans trans cis trans 1997 1999 trans trans 1995 2003 2000 2003 2003 2003 2003 2004 2004 The scission of PGCs from the TGN 2000 2003 2002 2000 2003 2003 2006 2003 2005 2 2003 2 2 2006 Fig. 2 a red line 1  2 b red line  2003 2004 Movement of PGCs from the Golgi complex to the target membrane 2000 2003 2005 1999 2000 2005 2000 2001 2000 2003 1999 2006 1999 2000 1998 2003 2004 2006 2004 2004 2001 Intermediate stations in post-Golgi transport 1995 2001 2004 2005 2003 2003 2005 2004 2005 2007 2004 Conclusions 2004 2006 2004 2006