1 Introduction [1] + − [2–4] [5] + + + + [6–8] [9,5] [10] [11,3] [3,12,13] [14–17] Clostridium botulinum [17,15,18] Lck [15,17] [15] [15] [15,19] [18] [10,18,20,21] [18] [22–24] 2 Materials and methods 2.1 Mice −/− [10,18] 2.2 Flow cytometric analysis − − 2.3 RNA extraction −/− + − − − + ® 2.4 Microarray ® www.Affymetrix.com −/− 2.5 Real-time RT-PCR analysis E u ( ct uc - ct us ) E r ( ct rc - ct rs ) E 3 Results and discussion [18] Fig. 1 Fig. 1 −/− Figs. 1 Fig. 1 [18] [25–27] [18] Supplementary Fig. 1 Fig. 2 Supplementary Fig. 1 [28–30] Fig. 2 Fig. 2 CD2–C [31] [31–33] [34–38] −/− −/− −/− Fig. 2 −/− −/− −/− Fig. 3 −/− −/− Supplementary Fig. 2 −/− −/− −/− −/− Fig. 3 −/− −/− Supplementary Fig. 3 Supplementary Fig. 3 [39–42] [19] [43,42,44,45] −/− Supplementary Fig. 4 −/− −/− RPS6 −/− [13] Fig. 4 low high high low −/− Supplementary Fig. 4 −/− [46,36] [47–49] [50,51] −/− Appendix A Supplementary data doi:10.1016/j.febslet.2007.07.077 Appendix A Supplementary data Supplementary figure 1 List of total genes downregulated or upregulated in CD2–C3 DN 3 thymocytes.  Supplementary figure 2 −/−  Supplementary figure 3 List of genes that are uniquely downregulated in CD2–C3 DN3 thymocytes.  Supplementary figure 4 −/−