1 Introduction [2,3] [3–6] [7] [8–12] [13–16] [17,18] [19] [20] [17,21–23] [24] [25] [1,26–28] [1] [1] 2 Materials and methods 2.1 Cell culture, transient transfections and cell stimulation −/− −/− −/− [1] [1] [29] 2.2 sIgM staining 6 All results shown are representative of at two to four independent experiments unless otherwise indicated. 3 Results 3.1 Loss of HSP27 phosphorylation in DT40 B cells lacking expression of PKD family kinases [1] −/− Fig. 1 −/− [1] [24] Fig. 1 Fig. 1 Fig. 1 Fig. 1 Fig. 1 3.2 Cellular proliferation and survival in DT40 B cells lacking expression of PKD family kinases [20] −/− +ve −/− Fig. 2 −/− −/− −/− −/− [17,30–32] Fig. 2 2 2 3.3 Antigen receptor regulated signalling pathways in PKD-null DT40 B cells −/− −/− +ve Fig. 3 −/− Fig. 3 Fig. 3 −/− Fig. 3 [33–35] −/− + −/− −/− + −/− [36] Fig. 3 −/− −/− [17,21,23,30,37] [38] [39,40] Fig. 4 −/− −/− Fig. 4 −/− Fig. 4 4 Discussion [41] [42] [20,23]