Introduction 1 2 3 4 5 5 6 7 8 p 9 10 11 6 12 13 14 15 Since there is an increasing interest in consuming products naturally rich in phytochemicals able to provide health benefits and prevent a wide variety of diseases, in this context we have investigated in the present work the effects of diet supplementation for 5 weeks with different types of cereal fractions naturally rich in polyphenols on certain parameters of immune function and redox state of peritoneal leukocytes from adult healthy mice. Material and methods Animals Mus musculus Experimental groups n Cereal products and preparation of extracts 2 2 Analysis of total polyphenols and antioxidant activity 16 r 2 Preparation of diets 1 1 Table 1 Nutritional composition of standard (control) and experimental (B, C, D and E) diets a Control B C D E HF b HF b b b HF b Polyphenols   Gallic acid — — — — 0.49 — — — —   Catechin — — — 10.8 0.19 2.04 3.34 — — p — 0.27 0.99 — 0.78 — — 1.04 —   Vanillic acid — 0.54 13.19 1.82 — 1.93 — 0.86 0.46 p — — — 0.21 0.16 1.28 18.91 0.06 0.68   Sinapic acid — — — — 0.45 4.73 — — —   Ferulic acid — 25.97 55.60 0.42 0.09 11.75 69.19 23.83 63.06   Quercetin — — a — a — — — —   Rutin — — a — a — — — —   Oryzanol  — — — — — a — — Vitamins   Vitamin A 0.45 — — — —   Vitamin D3 0.0038 — — — —   Vitamin E 2 13.65 0.2 1.33 5.55   Thiamin 0.1 1.87 0.41 1.81 1.48   Riboflavin 0.4 — — — —   Niacin 2 — — — —   Pantothenic acid 1.5 — — — —   Pyridoxin 0.4 0.87 0.2 0.18 0.68   Biotin 0.01 — — — —   Folacin 0.1 291 45 69 103 c 2 — — — —   Choline 50 — — — — Fatty acids   Palmitic acid 460 — — — —   Palmitoleic acid 39 — — — —   Stearic acid 130 — — — —   Oleic acid 650 — — — —   Linoleic acid 1,390 — — — —   Linolenic acid 130 — — — — Minerals   Calcium 880 25 10 78 62   Phosphorum 590 — — — —   Chloride 310 — — — —   Sodium 250 — — — —   Potasium 600 — — — —   Magnesium 140 259 174 910 203 Proteins d 15.4 33.6 11.5 21.3 16.85 Carbohidrates d 101.5 21.1 67.4 7.4 28 Fibre d 3.9 7.7 3.1 40.4 29.3 p p a b  c  d Collection of peritoneal leukocytes 17 14 18 Leukocyte functions 14 14 2 ·− 17 2 ·− 6 14 Leukocyte redox state 17 6 19 6 2 2 6 20 6 2 4 3 4 2 4 6 Statistical analysis P Results Immune system response 1 P P 1a 1b P P P P P P P 1c P P P 1d P 2 ·− P P 2 ·− P P P Fig. 1 a b c d P P P  Cellular redox state 2 P 2a 2b P P P P P P 2c P P P 2d 2e P Fig. 2 a b c d e P P P  Discussion 21 4 15 22 Urtica dioica O O O 13 23 p p 4 24 ® 25 26 27 28 29 p p 30 p −7 −4 31 23 32 33 34 p p 35 p 36 37 p 38 39 40 41 42 43 13 14 15 44 45 46 47 48 42 49 50