1 Introduction [1–4] [5] [6–11] [12–14] [15] [16] [17] mrc1Δ mrc1Δ [17] mrc1Δ [18] [19–21] [20–22] [22] mrc1 AQ [18,20,21] mrc1 AQ [18] cdc13-1 mrc1Δ [17] [23] tlc1 mrc1 cdc13-1 [23] cdc13-1 yku70Δ 2 Materials and methods 2.1 Yeast strains and plasmids RAD5 ade2-1 Supplemental Table 1 Supplemental Table 1 [24] cdc13-1 [25] [26] MRC1 mrc1 AQ URA3 [17,18] MRC1 MRC1 KanMX6 [27] MRC1 KanMX [27] KanMX MRC1 MRC1 MRC1 URA3 MRC1 MRC1 MRC1 URA3 URA3 MRC1 URA3 MRC1 MRC1 2.2 Spot tests 7 MRC1 mrc1 AQ 2.3 Yeast transformation [28] [29] 2.4 Western blots [30,31] [32] [33] 2.5 Telomere length measurement by Southern blot [34] 2.6 Synchronous cultures cdc13-1 cdc15-2 bar1 Supplemental Table 1 6 [13] [35] 2.7 Asynchronous cultures cdc13-1 7 7 2.8 Cell cycle position determination cdc13-1 cdc15-2 [36] 3 Results 3.1 cdc13-1 yku70Δ cdc13-1 [12,37,38] cdc13-1 [39–41] cdc13-1 [42] Fig. 1 MRC1 cdc13-1 cdc13-1 mre11Δ cdc13-1 rad50Δ cdc13-1 mrc1Δ Supplementary Fig. 1 cdc13-1 cdc13-1 mrc1Δ yku70Δ [43] YKU70 [36] Fig. 1 MRC1 yku70Δ yku70Δ [19–21,44] cdc13-1 TOF1 CSM3 cdc13-1 MRC1 Fig. 1 MRC1 3.2 cdc13-1 mrc1Δ [17–21] cdc13-1 mrc1Δ cdc13-1 mrc1Δ MRC1 mrc1 AQ Fig. 2 mrc1Δ cdc13-1 MRC1 mrc1 AQ cdc13-1 cdc13-1 mrc1Δ mrc1 AQ MRC1 MRC1 mrc1 AQ Fig. 2 3.3 cdc13-1 mrc1Δ yku70Δ mrc1Δ EXO1 cdc13-1 yku70Δ [36,39] cdc13-1 mrc1Δ yku70Δ mrc1Δ Fig. 3 cdc13-1 mrc1Δ exo1Δ cdc13-1 mrc1Δ cdc13-1 mrc1Δ EXO1 mrc1Δ yku70Δ Fig. 3 3.4 cdc13-1 mrc1Δ yku70Δ mrc1Δ mrc1Δ cdc13-1 mrc1Δ yku70Δ mrc1Δ mrc1Δ rad9Δ, rad17Δ rad24Δ [24] mrc1Δ mrc1Δ rad53Δ chk1Δ cdc13-1 [36,45,46] RAD53 CHK1 cdc13-1 mrc1Δ RAD53 SML1 [47] [48] RAD53 SML1 cdc13-1 mrc1 Fig. 4 SML1 cdc13-1 mrc1 sml1 MRC1 Fig. 4 cdc13-1 mrc1Δ cdc13-1 mrc1Δ cdc13-1 mrc1Δ Fig. 4 cdc13-1 mrc1Δ CHK1 yku70Δ mrc1Δ Fig. 4 yku70Δ [36] mrc1Δ yku70Δ yku70Δ chk1Δ yku70Δ mrc1Δ cdc13-1 mrc1Δ yku70Δ [36,45] 3.5 cdc13-1 cdc13-1 cdc13-1 cdc13-1 Fig. 5 cdc13-1 chk1Δ cdc13-1 rad9Δ cdc13-1 rad53Δ Fig. 5 cdc13-1 cdc13-1 mrc1Δ cdc13-1 cdc13-1 mrc1Δ Fig. 5 cdc13-1 cdc13-1 [45,46] cdc13-1 mrc1Δ cdc13-1 mrc1Δ chk1Δ cdc13-1 mrc1Δ rad53Δ mrc1Δ cdc13-1 cdc15-2 bar1 [13,39,40,45] bar1 cdc13-1 cdc15-2 bar1 cdc13-1- cdc13-1 rad9Δ cdc15-2 bar1 cdc15-2 cdc15-2 cdc13-1 cdc13-1 cdc15-2 bar1 Fig. 5 cdc13-1 rad9Δ cdc13-1 cdc13-1 mrc1Δ cdc13-1 cdc13-1 mrc1Δ exo1Δ Fig. 5 cdc13-1 exo1Δ [39] cdc13-1 mrc1Δ exo1Δ cdc13-1 exo1Δ cdc13-1 cdc13-1 mrc1Δ cdc13-1 rad9Δ Fig. 5 [23] cdc13-1 3.6 Mrc1 contributes to telomere length regulation Fig. 6 mrc1Δ rad50Δ yku70Δ cdc13-1 mrc1Δ cdc13-1 rad50Δ Supplementary Fig. 1 [49–52] 3.7 Mrc1 protects telomeres from extended ssDNA accumulation PDA1 cdc13-1 Fig. 6 cdc13-1 Fig. 5 cdc13-1 mrc1Δ cdc13-1 rad9Δ PDA1 cdc13-1 Fig. 6 [39] cdc13-1 cdc13-1 mrc1Δ yku70Δ mrc1Δ [53] cdc13-1 mrc1Δ exo1Δ cdc13-1 mrc1Δ cdc13-1 mrc1Δ yku70Δ mrc1Δ Fig. 3 4 Discussion cdc13-1 [39,40] [36,42,54,55] cdc13-1 yku70Δ [53] cdc13-1 mrc1Δ yku70Δ mrc1Δ cdc13-1 mrc1Δ EXO1 [17,18,22] [22] [15,56,57] mrc1Δ cdc17/pol1 cdc44/rfc1 [49] mrc1 S. cerevisiae cdc17/pol1 [50,51] [10,52] [36,39] [58] cdc13-1 cdc13-1 [59] Appendix A Supplementary data doi:10.1016/j.dnarep.2007.05.010 Appendix A Supplementary data