Introduction 2003 in planta 2004 2002 Leuconostoc mesenteroides 1954 L. mesenteroides 2000 C 2000 1992 Penicillium 2002 2003) 2003 2001 2003 2006 2005a b Materials and methods Asr 1989 Silene pratensis 1995 Asr Asr L. mesenteroides 2000 Asr Sma CCCGGG Nru TCGCGA Pfu Sma Nru Asr Asr Asr Sac Sal 1998 1 Fig. 1 Schematic representation of pPFA binary vector used for potato plant transformation  Transformation and regeneration of potato plants Agrobacterium tumefaciens 2000 Agrobacterium- 2005a Starch isolation Potato tubers were peeled and homogenized in a Sanamat Rotor (Spangenberg, The Netherlands). The resulting homogenate was allowed to settle overnight at 4°C and the potato juice was decanted and stored at −20°C for characterization of soluble alternan. The starch pellet was washed three times with water, air-dried at room temperature for at least three days and stored at room temperature. Immunological detection of alternans in tuber juices and gelatinized starches 2005a BI  2002 1982  Asr 1994 2005a AsrRT Asr 2000 GBSSI 2005 GBSSI Determination of morphological and physicochemical starch properties 2005a 50 2005a Results Detection of alternan in transgenic potato juices Asr 2001 1 1989 Asr Sma P G 2000 208 H 221 G 1092 S Agrobacterium Asr 2002 −1 2 −1  −1  Asr Ubi3 1994 3 Asr Asr Asr Fig. 2 −1  Fig. 3 Asr Ubi3 Asr  Alternan accumulation does not interfere with plant, tuber and starch morphologies Asr Asr 4 4 4 4 Fig. 4 A E B F C G D H 1996  The physicochemical properties and starch content of KDA transformants remain unchanged 50 T 0 H 1 Table 1 50 o H Transformants 50 T 0 H Amylose content(%) Starch content (mg/g FW) KD-UT 26.5 (±0.3) 67.9 (±0.1) 14.5 (±0.1) 22.3 (±0.2) 214.8 (±117.5) KDA1 (−) 24.4 (±0.2) 68.1 (±0.1) 17.0 (±0.1) 22.2 (±0.2) 103.4 (±66.3) KDA24 (−) 25.0 (±0.2) 68.0 (±0.1) 16.3 (±1.2) 21.3 (±0.4) 86.7 (±41.9) KDA16 (+) 24.9 (±0.3) 67.9 (±0.2) 16.4 (±1.3) 22.2 (±0.1) 140.0 (±88.2) KDA19 (+) 27.9 (±0.2) 67.7 (±0.0) 15.2 (±0.1) 23.0 (±0.2) 137.1 (±38.2) KDA27 (+) 22.8 (±0.7) 67.7 (±0.2) 16.2 (±0.5) 22.2 (±0.4) 289.3 (±39.7) KDA13 (++) 24.0 (±0.1) 67.8 (±0.1) 16.0 (±0.7) 22.2 (±0.5) 107.2 (±49.4) * Median value of the granule size distribution † Temperature of onset of starch gelatinization ‡ Enthalpy released AGPase GBSSI SuSy AGPase SSIII SBEI GBSSI 5 AGPase GBSSI AGPase GBSSI AGPase −1 −1  GBSSI GBSSI 1 GBSSI GBSSI Fig. 5 SuSy AGPase SSIII SBEI GBSSI Ubi3 GBSSI GBSSI  Discussion 2005a 2005b −1  −1  2005a 2006 Streptococcus downei 2005b 2003 2006 2005a b 2003 4 4 2000 −1  2001 2003 −1  −1