1 Introduction 2+ [1] [2,3] [2] [1] [1,4] [1] [5] [1,6] [7–9] [9] [10] [11] [12] [13] [14,15] [16] [17] [18] Xenopus [19] [20,21] [21] 2 2 [22–25] 2 2 2 Materials and methods 2.1 Primary culture of neonatal rat cardiac myocytes and preparation of polysomes [26] [27] 6 2 2 [25] 2.2 RNA preparation and quantitative PCR (qPCR) [22,25] [25] Table 1 2.3 Western blotting [28] 6 [29] 3 Results 3.1 ET-1 regulates expression of multiple klf family members in cardiac myocytes [25] Table 2 Fig. 1 Fig. 1 Fig. 1 Fig. 1 Fig. 1 Fig. 1 [1,4,6] 3.2 Regulation of Klf2, Klf4, Klf5 and Klf6 by ET-1 Fig. 2 [30,31] Fig. 2 [32] [33–35] [24] n n n n Fig. 3 [36] [37] [34] Fig. 3 [38] Fig. 2 Fig. 3 3.3 Translation-state analysis of Klf2, Klf4, Klf5 and Klf6 mRNA expression [39,40] Fig. 4 Fig. 4 Fig. 4 Fig. 4 Fig. 2 Fig. 4 Fig. 2 Fig. 4 3.4 2 2 2 2 [41] [42] Fig. 5 Fig. 1 [23] 2 2 Fig. 5 2 2 [43] 2 2 [42] 2 2 Fig. 5 2 2 Fig. 5 Fig. 5 [15] Fig. 6 Fig. 3 Fig. 6 4 Discussion 4.1 Regulation of gene expression by Klf family members [14] [8] Fig. 1 Fig. 2 [1,6] Table 2 Fig. 1 [44,45] 4.2 Regulation of expression of Klf2, Klf4, Klf5 and Klf6 Fig. 1 Fig. 2 Fig. 3 2 2 Fig. 5 [36] Fig. 3 [46] [24] [47] [47] Fig. 3 [48] [49] Fig. 1 Figs. 3A and 5D Fig. 2 12,14 2 [50] [51] [52,53] Fig. 2 Fig. 3 [54] [55] Fig. 4 [56] 254 Fig. 4 Fig. 4 2 2 4.3 Regulating Klf-dependent gene expression in cardiac myocytes [21] Fig. 1 Fig. 1 2 2 Fig. 5 [23] Fig. 6