Introduction 2004 Streptomyces mobaraensis 2004 2004 2005 Corynebacterium glutamicum Corynebacterium ammoniagenes 2005 C. glutamicum 2003 2003 2003 1994 2005 C. glutamicum 1995 1992 1997 C. glutamicum 2003  2004 2003 C. glutamicum 2006 C. glutamicum Chryseobacterium proteolyticum C. glutamicum 2006 2007 C. glutamicum C. ammoniagenes Materials and methods Bacterial strains, plasmids, and culture medium 1 2003 cspB C. glutamicum C. ammoniagenes S. mobaraensis 2003 C. glutamicum 2003 Corynebacterium 4 2 4 2 4 2 4 4 2 4 2 3 2003 Table 1 Pro-TG accumulation by coryneform bacteria carrying pPSPTG1 in MMTG medium at 30°C for 72 h Strain Pro-TG (mg/l) Corynebacterium ammoniagenes 454 Corynebacterium glutamicum 147 Brevibacterium taipei 201 Corynebacterium glutamicum Micrococcus glutamicus 66 Brevibacterium roseum 269 Corynebacterium glutamicum Brevibacterium flavum 314 Corynebacterium herculis 54 Corynebacterium glutamicum Brevibacterium lactofermentum 118 Corynebacterium acetoacidophilum 369 Corynebacterium glutamicum Brevibacterium divaricatum 391 Brevibacterium saccharolyticum 238 Brevibacterium immariophilium 257 Microbacterium ammoniaphilum 423 Corynebacterium glutamicum Corynebacterium lilium 246 Corynebacterium callunae 759 Brevibacterium thiogenitalis 156 Jar fermentation v v 4 2 4 2 4 2 4 4 2 4 2 Protein analysis 1970 2002 1997 2002 N 2002 N 1997 Results and discussion C. ammoniagenes 1 1 1 C. ammoniagenes Corynebacterium acetoacidophilum C. glutamicum Brevibacterium divaricatum Microbacterium ammoniaphilum Corynebacterium callunae Fig. 1 a Lane 1 lanes 2 3 B. saccharolyticum lanes 4 5 B. roseum lanes 6 7 B. immariophilium lanes 8 9 C. glutamicum lanes 10 11 C. glutamicum lanes 12 13 C. callunae lanes 14 15 C. herculis lanes 16 17 C. acetoacidophilum lanes 18 C. ammoniagenes b Lanes 1 2 C. glutamicum lane 3 lanes 4 5 C. glutamicum lanes 6 7 M. ammoniaphilum lanes 8 9 B. taipei lanes 10 11 B. thiogenitalis lanes 12 13 C. glutamicum lanes 14 15 C. glutamicum lane 16 C. ammoniagenes  S. mobaraensis 1 1 C. glutamicum C. glutamicum 2006 2003 1 Effect of signal peptide sequence on pro-TG secretion C. ammoniagenes 2 C. glutamicum 2 C. ammoniagenes C. acetoacidophilum C. glutamicum M. ammoniaphilum C. callunae C. ammoniagenes C. glutamicum C. glutamicum 2 C. acetoacidophilum C. glutamicum M. ammoniaphilum C. callunae C. ammoniagenes Fig. 2 a C. ammoniagenes C. glutamicum black gray backgrounds b solid bars C. ammoniagenes open bars C. glutamicum  C. ammoniagenes C. glutamicum 2 C. glutamicum C. ammoniagenes C. glutamicum 2003 C. ammoniagenes C. ammoniagenes Materials and methods 3 N 2003 C. ammoniagenes Streptomyces albogriseolus 2003 C. ammoniagenes 2003 Fig. 3 C. ammoniagenes open circles open squares closed triangles  C. ammoniagenes C. ammoniagenes