The effect of daily dietary supplements of an antioxidant mixture (AM) consisting of beta carotene, alpha tocopherol, ascorbic acid, rutin, selenium, and zinc on the survival of male C57BL/6 mice starting at 2, 9, 16, and 23 months of age was investigated. The survival of mice given AM starting at 2 and 9 months of age was found to increase significantly (from 86 to 108 days) compared to the control. The times, of 50, 90, and 100% mortality in mice given AM starting at 2 and 9 months of age increased by 16-9.5% compared to the control, whereas in mice given AM, starting at 16 and 23 months of age, no effect was observed.