We studied the effect of herbicide glyphosate on fertility and lifespan of D. melanogaster. Wild type flies obtained from the natural population and maintained in the laboratory and flies of the Oregon-R strain were used. Glyphosate concentrations 0.028, 0.28, and 2.8 mg/ml were tested. Glyphosate in a concentration of 2.8 mg/ml reduced lifespan of male flies and the number of pupae and imago in the progeny of D. melanogaster from natural population and Oregon-R strain, while the concentrations of 0.028 and 0.28 mg/ml produced no adverse effect on the studied parameters.