Conscientiousness is the strongest personality predictor of longevity. The present study examined which facets of conscientiousness are the most strongly related to mortality risk in a large longitudinal sample of middle-aged and older adults. Seven-year mortality data were obtained from participants (total N > 11,000) from the Health and Retirement Study (HRS, 2008-2014). Six facets of conscientiousness, demographic factors, disease burden, smoking and physical inactivity were assessed at baseline. Controlling for demographic factors, every standard deviation higher order, traditionalism, virtue, and responsibility was related to an approximately 10% reduced risk of mortality; industriousness was associated with an almost 25% lower likelihood of mortality. Except for traditionalism, these associations were partially accounted by health and behavioral covariates. Self-control was not related to longevity. When all facets and the covariates were simultaneously included, only industriousness was significantly associated with mortality. The present study provides new evidence about the specific facets of conscientiousness that are related to longevity.