The discrepancies were found in some physiological properties of enterococcal strains isolated from gastrointestinal tract of long-livers in 1978-1981 years after long-term storage in lyophilized state. Different results of conventional tube tests obtained before and after long-term storage concerning fermentation of glycerol (38% strains), sucrose (36%), manitol (33%), sorbitol (24%), raffinose (27%), lactose, inulin, xylose (3%) were obtained. When comparing results obtained with the help of API-systems with the data of conventional tube tests it was determined that the discrepancies were most frequently observed in carbohydrate fermentation tests for glycerol (27% cases), mannitol (25%), sorbitol (14%), sucrose (11%), raffinose (12%) and rhamnose (10%). Discrepancies in carbohydrate fermentation tests for lactose, inulin, dulcitol, sorbose, xilose and salicin were observed only in 1% of cases.