The sensitivity of harpacticoid copepods was tested against selected pollutants. Acute toxicity tests were carried out for five hyporheic species exposed to pesticides, ammonia, and metals. The stygoxene Bryocamptus zschokkei, B. minutus, B. pygmaeus and Attheyella crassa; and the stygophilous B. echinatus were sampled and cultured during 8 months in controlled conditions. A first test protocol is presented. The acute endpoints among species fell within one order of magnitude. The sensitivity among various species evaluated in this study is consistent and the choice of species for further sediment/groundwater assessment is not specific to a chemical class. These potential test organisms would be more suitable to protect meiofaunal communities.